Composite

Part:BBa_K1053302:Design

Designed by: Nana Hirose   Group: iGEM13_Tokyo-NoKoGen   (2013-09-10)


λPR-RBS-lacI-DT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1222
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBa_K1053302 (λPR-RBS-cI-DT) is based on promoter (lambda cI regulated) and lacI repressor from E. coli (+LVA). This is one of the parts contained in Repressilator. The lacI repressor constitutively expresses, but it is inhibited when cI repressor from the Elowitz's repressilator expresses.

Source

lacI repressor is from E. coli.

References

A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000